截頭肽庫（Truncation Peptide Library）

用以確定抗原表位的最短長度，通過系統(tǒng)性地截取兩側(cè)末端氨基酸獲得。一般與丙氨酸篩查肽庫結(jié)合使用，在使用丙氨酸確定表位關(guān)鍵殘基后，截頭肽庫用來進(jìn)一步確定最短表位序列及其作用。

在許多案例中，截頭肽庫用于可鑒定篩選增強(qiáng)蛋白水解作用的多肽。它可以作為研究多肽藥物代謝降解程度的工具。

應(yīng)用領(lǐng)域

參考文獻(xiàn)

Svenson J, Stensen W, Brandsdal BO, Haug BE, Monrad J, and Svendsen JS. Antimicrobial peptides with stability toward tryptic degradation. Biochemistry. Mar 2008 25; 47(12): 3777-88.



Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, and Houslay MD. Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5. Biochem. J. Aug 2006; 15; 398(1): 23-36.



Ostermeier M, Nixon AE, Shim JH, and Benkovic SJ. Combinatorial protein engineering by incremental truncation. Proc. Natl. Acad. Sci. U S A. Mar 1999 30; 96(7): 3562-7.



de Figueiredo P, Roberts RL, and Nester EW. DARTs: A DNA-based in vitro polypeptide display technology. Proteomics. Oct 2004; 4(10): 3128-40.



Horswill AR, Naumann TA, and Benkovic SJ. Using incremental truncation to create libraries of hybrid enzymes. Methods Enzymol. 2004; 388: 50-60.