主要特點

高特異性

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• 特異性識別目標Payload分子
• 與其它Payload分子無交叉反應
• 可結合游離型和偶聯型Payload

高質量

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• 經兩種LBA方法驗證，都有良好表現
• 標曲檢測范圍寬，線性好，靈敏度高
• 適用于偶聯抗體和總抗體檢測方法開發

充分驗證

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• 產品應用驗證參照ICH M10指南
• 通過食蟹猴血清最小稀釋倍數測試

ADC藥代動力學分析

藥代動力學主要研究機體對藥物吸收、分布、代謝和排泄（ADME）過程中體內藥物濃度隨時間變化的規律。ADC結構復雜，包含單克隆抗體，細胞毒性分子（Payload）和化學連接子，通常其藥代動力學分析需檢測以下幾個指標，包括：

總抗體，即小分子毒素抗體比（drug-antibody ratio, DAR）大于或等于 0 的 ADC

偶聯抗體，即 DAR 大于或等于 1 的 ADC

游離Payload或其代謝物

ELISA定量分析法通常用于測定總抗體和偶聯抗體濃度。如圖 2 所示，多種形式可用于檢測目標分析物。

圖2. ELISA法ADC PK分析示意圖

產品列表

金斯瑞提供多種抗Payload抗體、抗獨特型抗體、抗人 IgG Fc 抗體和重組靶抗原，用于ELISA 分析中測定ADC偶聯抗體和總抗體濃度。

• 抗Payload抗體

  產品編號	名稱	數量	價格	操作
  A02217	DXd Antibody (39F4), mAb, Mouse		￥1600.00 40 μg
  A02223	DM1 Antibody (15L2), mAb, Mouse		￥1600.00 40 μg
  A02224	MMAE Antibody (69F7), mAb, Mouse		￥1600.00 40 μg
  A02225	MMAF Antibody (11B8), mAb, Mouse		￥1600.00 40 μg

• 抗獨特型抗體
  抗Cetuximab/Erbitux
  (靶向EGF Receptor)

  抗Daratumumab/Darzalex
  (靶向CD38)

  抗Obinutuzumab/Gazyva
  (靶向CD20)

  抗Panitumumab/Vectibix
  (靶向EGF Receptor)

  抗Pertuzumab/Perjeta
  (靶向?HER2)

  抗Rituximab/Rituxan
  (靶向?CD20)

  抗Trastuzumab/Herceptin
  (靶向HER2)

  *查看 更多 抗獨特型抗體！

  抗Cetuximab/Erbitux (靶向EGF Receptor)

  產品編號	名稱	數量	價格	操作
  A01938	Anti-Cetuximab Antibody (18D5), mAb, Mouse		￥990.00 40 μg
  A01939	Anti-Cetuximab Antibody (20H1), mAb, Mouse		￥990.00 40 μg
  A01991	MonoRab? Anti-Cetuximab Antibody (69H10), mAb, Rabbit		￥1980.00 40 μg
  A01992	MonoRab? Anti-Cetuximab Antibody (90G3), mAb, Rabbit		￥1980.00 40 μg

  抗Daratumumab/Darzalex (靶向CD38)

  產品編號	名稱	數量	價格	操作
  A01996	Anti-Daratumumab Antibody (11F2), mAb, Mouse		￥990.00 40 μg
  A01997	Anti-Daratumumab Antibody (6A3), mAb, Mouse		￥990.00 40 μg

  抗Obinutuzumab/Gazyva (靶向CD20)

  產品編號	名稱	數量	價格	操作
  A01945	Anti-Obinutuzumab Antibody (18H8), mAb, Mouse		￥990.00 40 μg
  A01946	Anti-Obinutuzumab Antibody (16B7), mAb, Mouse		￥990.00 40 μg
  A01966	MonoRab? Anti-Obinutuzumab Antibody (8G12), mAb, Rabbit		￥1980.00 40 μg
  A01967	MonoRab? Anti-Obinutuzumab Antibody (169F10), mAb, Rabbit		￥1980.00 40 μg

  抗Panitumumab/Vectibix (靶向EGF Receptor)

  產品編號	名稱	數量	價格	操作
  A02099	Anti-Panitumumab Antibody (2E7), mAb, Mouse		￥990.00 40 μg
  A02100	Anti-Panitumumab Antibody (9G12), mAb, Mouse		￥990.00 40 μg

  抗Pertuzumab/Perjeta (靶向?HER2)

  產品編號	名稱	數量	價格	操作
  A02150	Anti-Pertuzumab Antibody (1A3), mAb, Mouse		￥990.00 40 μg
  A02151	Anti-Pertuzumab Antibody (11H4), mAb, Mouse		￥990.00 40 μg

  抗Rituximab/Rituxan (靶向?CD20)

  產品編號	名稱	數量	價格	操作
  A01942	MonoRab? Anti-Rituximab Antibody (137C6), mAb, Rabbit		￥1980.00 40 μg
  A01943	MonoRab? Anti-Rituximab Antibody (194D7), mAb, Rabbit		￥1980.00 40 μg
  A01969	Anti-Rituximab Antibody (6C1), mAb, Mouse		￥990.00 40 μg
  A01970	Anti-Rituximab Antibody (17B6), mAb, Mouse		￥990.00 40 μg

  抗Trastuzumab/Herceptin (靶向HER2)

  產品編號	名稱	數量	價格	操作
  A02032	Anti-Trastuzumab Antibody (11C4), mAb, Mouse		￥1980.00 40 μg
  A02033	Anti-Trastuzumab Antibody (15H2), mAb, Mouse		￥1980.00 40 μg

• 抗人IgG Fc抗體

  產品編號	名稱	數量	價格	操作
  A01854	Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb		￥990.00 200 μl

• 重組靶點蛋白

  產品編號	名稱	數量	價格	操作
  Z02731	BCMA, Human		￥1260.00 20 μg 1 mg
  Z03194	EGFR, His, Human		￥600.00 10 μg 50 μg 1 mg
  Z03504	CLDN18.2, His, Human		￥2200.00 10 μg 100 μg 1 mg
  Z03642	CD30, His, Human		￥1600.00 100 μg 1 mg
  Z03643	Siglec-2/CD22, hFc, Human		￥1760.00 100 μg

應用案例

• 抗Payload抗體和抗獨特型抗體測定偶聯抗體濃度
  

  圖3. 抗獨特型抗體和抗DXd抗體夾心法ELISA測量DXd偶聯曲妥珠單抗標準曲線。實驗采用偶聯抗體測定形式2。

  The Sandwich ELISA assay was developed using Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033) and DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) and as the capture and detection antibodies, respectively.

  In this ELISA assay, DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) was labeled with HRP. GenScript can provide customized conjugation service for this product per customer's request.

  Capture antibody: Anti-Trastuzumab Antibody (15H2), mAb, Mouse, 2 μg/ml
  Detection antibody: DXd Antibody (39F4), mAb, Mouse, 0.22 μg/ml.

• 抗Payload抗體和抗人IgG Fc抗體測定偶聯抗體濃度
  

  圖4. 抗人IgG Fc抗體和抗DXd抗體夾心法ELISA測量猴血清中DXd偶聯曲妥珠單抗濃度標準曲線。實驗采用偶聯抗體測定形式1。

  The DXd conjugated Trastuzumab Sandwich ELISA assay was developed using DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) as the capture and detection antibodies, respectively.

  The samples tested in this assay were DXd conjugated Trastuzumab. The control samples were not serum spiked. The monkey serum samples were spiked with cynomolgus monkey serum matrix.

• 抗獨特型抗體抗體對測定總抗體濃度
  

  圖5. 曲妥珠單抗夾心法ELISA測量標準曲線。曲妥珠單抗夾心法ELISA分別使用Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032-40)和Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033-40)作為捕獲抗體和檢測抗體。實驗采用總抗體測定形式4。

  In this ELISA assay, Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033-40) was labeled with Biotin.

  GenScript can provide customized conjugation services for this product per the customer's request. The sensitivity of detecting Trastuzumab was observed to be up to 61 pg/ml.