| 產品編號 | 名稱 | 數量 | 價格 | 操作 |
|---|---|---|---|---|
|
A00186
|
THE? His Tag Antibody, mAb, Mouse |
|
￥2500.00 100 μg |
|
基于以上的特性，THE™ Anti-His mAb在Western blot，Dot blot，ELISA，免疫熒光染色，以及流式細胞技術等方面有廣泛應用，并能取得良好的實驗效果。
| 產品說明 | 規格 | 溶解 | 存儲條件 |
|---|---|---|---|
| 抗His-tag的單克隆抗體可以用于鑒定、檢測和純化細菌、昆蟲細胞、哺乳動物細胞等表達系統表達的His融合蛋白。本公司以6xHis作為免疫原，獲得了高親和性和高特異性的單抗細胞株，以此制備小鼠腹水并通過protein G親和純化得到了THE™ Anti-His mAb（IgG1亞型）。 | 100μg凍干粉（緩沖液PBS pH7.4，含0.02%疊氮化鈉）。 | 用100μl去離子水溶解凍干物THE™ His mAb（100μg）終濃度為1mg/ml。 |
|
應用
由于影響合適工作濃度的條件包括：二抗濃度，監測方法的靈敏度，溫度及孵育時間等，所以實驗人員進行具體實驗時需要自行測定本產品的合適使用濃度。
使用本產品時的推薦濃度為：
注：在運輸和儲存過程中，可能會有少量的抗體粘附在包裝瓶口處。使用前需使用臺式離心機輕微離心以讓粘附的產品離心到包裝瓶的底部。
Figure 1：Sensitivity comparison of 6xHis-tag Fusion Protein by Western blot using His Antibody，mAb，Mouse from GenScript（A00186，0.1 μg/ml）and Qiagen（34698，0.1 μg/ml）.
Figure 2：Western blot analysis of N-terminal and C-terminal 6xHis-tag Fusion Protein using THE™ His Antibody，mAb，Mouse（A00186，1 μg/ml）.
Figure 3：Sensitivity comparison of 5xHis-GST in Whole Cell Lysates by Dot blot using His Antibody，mAb，Mouse from GenScript（A: A00186，1 μg/ml）and Qiagen（B: 34698，1 μg/ml and C:34660，1 μg/ml）.
Figure 4：Sensitivity comparison of 4xHis-GST in Whole Cell Lysates by Western blot using His Antibody，mAb，Mouse from GenScript（A00186，1 μg/ml）and Qiagen（34698 ，1 μg/ml）.
Figure 5：Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THE™ His Antibody，mAb，Mouse（A00186）.
Figure 6：Consistency analysis of THE™ His Antibody，mAb，Mouse（GenScript，A00186，1 μg/ml）with His-tagged fusion protein by Western blot.
Figure 7: Immunocytochemistry/Immunofluorescence analysis of HEK293 cells（A）and HEK293 cells transfected with His tagged fusion gene（B）using THE™ His Antibody，mAb，Mouse（A00186）.
Figure 8: Flow cytometric analysis of HEK293 cells（A）and HEK293 cells transfected with His tagged fusion gene（B）using THE™ His Antibody，mAb，Mouse（A00186）.
| 產品編號 | 名稱 | 數量 | 價格 | 操作 |
|---|---|---|---|---|
|
A00174
|
His-tag Antibody, pAb, Rabbit |
|
￥990.00 40 μg |
|
|
A00205
|
His-tag antibody [Biotin], pAb, Rabbit |
|
￥990.00 40 μg |
|
|
A00612
|
THE? His Tag Antibody [HRP], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A00613
|
THE? His Tag Antibody [Biotin], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01620
|
THE? His Tag Antibody [FITC], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01800
|
THE? His Tag Antibody [iFluor 488], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01801
|
THE? His Tag Antibody [iFluor 555], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01802
|
THE? His Tag Antibody [iFluor 647], mAb, Mouse |
|
￥2800.00 100 μg |
|
|
A01857
|
MonoRab? Anti-His Tag (C-term) Antibody(25B6E11), mAb, Rabbit |
|
￥1980.00 40 μg |
|
