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雜亂肽庫(Scrambled Library)

是可變性較高的肽庫,由原始序列的片段重新排列組合而成。亂序庫可以展示所有可能的肽取代結構,是理想的序列優化手段,可用于蛋白、抗體、基因等的靶向分子探針。雜亂肽庫可用作陰性對照。

應用領域

目的蛋白篩選

陰性對照

Overlapping Peptide
Alanine Scanning
Truncation Library
Positional Scanning
Random Library
Scrambled Library
T Cell Truncated Library
 

參考文獻

    Jiang QS, and Wang SQ. Design and screening of antisense oligodeoxynucleotides against PAI-1 mRNA in endothelial cells in vitro. Acta. Pharmacol. Sin. Aug 2006; 27(8): 1018-23.

    Oh JE, Hong SY, and Lee KH. Structure-activity relationship study: short antimicrobial peptides. J. Pept. Res. Jan 1999; 53(1): 41-6.

    Simon-Haldi M, Mantei N, Franke J, Voshol H, and Schachner M. Identification of a peptide mimic of the L2/HNK-1 carbohydrate epitope. J. Neurochem. Dec 2002; 83(6): 1380-8.

    Murayama O, Nishida H, and Sekiguchi K. Novel peptide ligands for integrin alpha 6 beta 1 selected from a phage display library. J. Biochem. Aug 1996; 120(2): 445-51.

    Keizer DW, Miles LA, Li F, Nair M, Anders RF, Coley AM, Foley M, and Norton RS. Structures of phage-display peptides that bind to the malarial surface protein, apical membrane antigen 1, and block erythrocyte invasion. Biochemistry. Aug 2003; 26; 42(33): 9915-23.

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