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Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection

Microbiol Res. 2024-05; 
Mianhuan Li, Yang Yang, Pusen Wang, Weitao Que, Lin Zhong, Zhao Cai, Yang Liu, Liang Yang, Yingxia Liu
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Proteins, Expression, Isolation and Analysis … Equal amounts of proteins were resolved on 4–12% Bis-Tris gradient gels (GenScript, nanjing, China) and then transferred onto nitrocellulose membranes. The membranes were … Get A Quote

摘要

The progress of viral infection involves numerous transcriptional regulatory events. The identification of the newly synthesized transcripts helps us to understand the replication mechanisms and pathogenesis of the virus. Here, we utilized a time-resolved technique called metabolic RNA labeling approach called thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-seq) to differentially elucidate the levels of steady-state and newly synthesized RNAs of BHK21 cell line in response to human coronavirus OC43 (HCoV-OC43) infection. Our results showed that the Wnt/β-catenin signaling pathway was significantly enriched with the newly synthesized transcripts of BHK21 cell line in response to HCoV-OC43 ... More

關(guān)鍵詞

HCoV-OC43, Introduction, Remdesivir, SLAM-seq, Viral infection, Wnt pathway
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