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Proteins, Expression, Isolation and Analysis> | … (Genscript) and the bound protein was eluted by 300 mM imidazole in a lysis buffer. The eluted protein was … The protein fractions were loaded onto a gel filtration in SEC buffer 2 (25 mM … | Get A Quote |
Mpox virus has wildly spread over 108 non-endemic regions in the world since May 2022. DNA replication of mpox is performed by DNA polymerase machinery F8-A22-E4, which is known as a great drug target. Brincidofovir and cidofovir are reported to have broad-spectrum antiviral activity against poxviruses, including mpox virus in animal models. However, the molecular mechanism is not understood. Here we report cryogenic electron microscopy structures of mpox viral F8-A22-E4 in complex with a DNA duplex, or dCTP and the DNA duplex, or cidofovir diphosphate and the DNA duplex at resolution of 3.22, 2.98 and 2.79??, respectively. Our structural work and DNA replication inhibition assays reveal that cidofovir diphos... More