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TMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice

Oxid Med Cell Longev. 2022-10; 
Xueyi Zhu, Baojun Liu, Zhenhui Ruan, Mengmeng Chen, Congcong Li, Hanlin Shi, Xi Huang, Hang Yu, Yaolong Zhou, Hehua Zhu, Jing Sun, Ying Wei, Weifang Xu, Jingcheng Dong
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Proteins, Expression, Isolation and Analysis … each well and separated by 10% SDS-PAGE, and then, the protein bands were electrotransferred onto 0.2μm PVDF membranes by the eBlot? L1 wet protein transfer system (GenScript Get A Quote

摘要

unassigned: Cycloastragenol (CAG) has been reported to alleviate airway inflammation in ovalbumin- (OVA-) induced asthmatic mice. However, its specific mechanisms remain unclear. unassigned: This study is aimed at investigating the effects of CAG on asthma, comparing its efficacy with dexamethasone (DEX), and elucidating the mechanism of CAG's regulation. unassigned: The asthma mouse model was induced by OVA. CAG at the optimal dose of 125?mg/kg was given every day from day 0 for 20-day prevention or from day 14 for a 7-day treatment. We observed the preventive and therapeutic effects of CAG in asthmatic mice by evaluating the airway inflammation, AHR, and mucus secretion. Lung proteins were used for TMT-base... More

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