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Preclinical Development of a Novel Epitope-based DNA Vaccine Candidate against SARS-CoV-2 and Evaluation of Immunogenicity in BALB/c Mice

AAPS PharmSciTech. 2024-03; 
Kanwal Khalid, Hui Xuan Lim, Ayaz Anwar, Soon Hao Tan, Jung Shan Hwang, Seng-Kai Ong, Chit Laa Poh
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Proteins, Expression, Isolation and Analysis … , M, and N proteins were cloned into the mammalian expression vector pVAX1 under the control … and denoted as pVAX1/S2-6EHGFP (GenScript, California, USA). The coding sequence … Get A Quote

摘要

The protective efficacies of current licensed vaccines against?COVID-19 have significantly reduced as a result of SARS-CoV-2 variants of concern?(VOCs) which carried multiple mutations in the Spike (S) protein. Considering that?these vaccines were developed based on the S protein of the original SARS-CoV-2?Wuhan strain, we designed a recombinant plasmid DNA vaccine based on highly?conserved and immunogenic B and T cell epitopes against SARS-CoV-2 Wuhan strain?and the Omicron VOC. Literature mining and bioinformatics were used to?identify 6 immunogenic peptides from conserved regions of the SARS-CoV-2 S and?membrane (M) proteins. Nucleotide sequences encoding these peptides representing?highly conserved... More

關鍵詞

DNA vaccine, SARS-CoV-2, VOCs, next-generation
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