国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

至今,GenScript的服務及產品已被Cell, Nature, Science, PNAS等1300多家生物醫藥類雜志引用近萬次,處于行業領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發表科研成果,再次證明GenScript 有能力幫助業內科學家Make research easy.

Construction of a grpE-based plasmid addiction system in Escherichia coli and its application in phloroglucinol biosynthesis

J Appl Microbiol. 2024-05; 
Ji-Ming Wang, Yu-Jin Cao, Xiao Men, Hai-Bo Zhang
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … A cloning plasmid pUC57S-grpEm carrying the synonymously re-encoded grpE expression cassette grpEm was purchased from Nanjing GenScript Biotech Co., Ltd. (Nanjing, China) (… Get A Quote

摘要

objective: Biotechnical processes in Escherichia coli often operate with artificial plasmids. However, these bioprocesses frequently encounter plasmid loss. To ensure stable expression of heterologous genes in E. coli BL21(DE3), a novel plasmid addiction system (PAS) was developed. results: This PAS employed an essential gene grpE encoding a cochaperone in the DnaK-DnaJ-GrpE chaperone system as the selection marker, which represented a chromosomal ΔgrpE mutant harboring episomal expression plasmids that carry supplementary grpE alleles to restore the deficiency. To demonstrate the feasibility of this system, it was implemented in phloroglucinol (PG) biosynthesis, manifesting improved host tolerance to PG and i... More

關鍵詞

grpE, essential gene, phloroglucinol, plasmid addiction system, tolerance, transcriptomic analysis
              主站蜘蛛池模板: 循化| 广安市| 海南省| 秦安县| 长汀县| 加查县| 遵义县| 修文县| 黄冈市| 吉木乃县| 桓仁| 巴东县| 望奎县| 江安县| 潍坊市| 泗水县| 阳东县| 界首市| 太仓市| 新竹县| 通辽市| 鄯善县| 安乡县| 波密县| 阳城县| 利川市| 明光市| 托克逊县| 彰武县| 白城市| 宁武县| 汝州市| 东海县| 广州市| 东城区| 左贡县| 彝良县| 垣曲县| 敦化市| 胶州市| 漯河市|