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Filtered Saliva for Rapid and Accurate Analyte Detection for POC Diagnostics

Diagnostics (Basel). 2024-05; 
Nadia Farsaeivahid, Christian Grenier, Ming L Wang
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … SARS-CoV-2 monoclonal S antibody and S protein of SARS-CoV-2 were purchased from Genscript. Gold nanoparticles (GNp, 10 nm diameter), PBS (0.1 mol/L, pH 7.4), thionine … Get A Quote

摘要

Saliva has shown considerable promise as a diagnostic medium for point-of-care (POC) and over-the-counter (OTC) diagnostic devices due to the non-invasive nature of its collection. However, a significant limitation of saliva-based detection is undesirable interference in a sensor's readout caused by interfering components in saliva. In this study, we develop standardized sample treatment procedures to eliminate bubbles and interfering molecules while preserving the sample's target molecules such as spike (S) protein and glucose. We then test the compatibility of the pretreatment system with our previously designed SARS-CoV-2 and glucose diagnostic biosensing systems for detecting S protein and glucose in subjec... More

關鍵詞

SARS-CoV-2 spike protein, diagnostics, electrochemical biosensor, glucose, point of care (POC) devices, saliva, saliva filtration process
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