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Modular multiwell viscoelastic hydrogel platform for two- and three-dimensional cell culture applications

biorxiv. 2023-10; 
Mackenzie L Skelton, James L Gentry, Leilani R Astrab, Joshua A Goedert, E Brynn Earl, Emily L Pham, Tanvi Bhat, Steven R Caliari
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Proteins, Expression, Isolation and Analysis … (GCKKK-Adamantane, Genscript). Viscoelastic hydrogels with … mM RGD peptide (GCGTGRGDSPG, Genscript) in all hydrogel … (GCKGGPQG↓IWGQGKCG, Genscript) replaced the DTT … Get A Quote

摘要

Hydrogels have gained significant popularity as model platforms to study the reciprocal interactions between cells and their microenvironment. While hydrogel tools to probe many characteristics of the extracellular space have been developed, fabrication approaches remain challenging and time-consuming, limiting multiplexing or widespread adoption. Thus, we have developed a modular fabrication approach to generate distinct hydrogel microenvironments within 96-well plates for increased throughput of fabrication as well as integration with existing high-throughput assay technologies. This approach enables hydrogel mechanical characterization and was used to generate both elastic and viscoelastic hydrogels across ... More

關鍵詞

disease modeling, high-throughput, hydrogels, multiwell plate
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