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Proteins, Expression, Isolation and Analysis> | … The supernatant was incubated with anti-DYKDDDDK G1 affinity resin (Genscript) for 4 h at 4C with mixing before washing the resin with VPS13 buffer four times. Chaperone removal … | Get A Quote |
ATG9A, a transmembrane protein of the core autophagy pathway, cycles between the Golgi, endosomes and a vesicular compartment. ATG9A was recently shown to act as a lipid scramblase, and this function is thought to require its interaction with another core autophagy protein, ATG2A, which acts as a lipid transfer protein. Together, ATG9A and ATG2A are proposed to function to expand the growing autophagosome. However, ATG9A is implicated in other pathways including membrane repair and lipid droplet homeostasis. To elucidate other ATG9A interactors within the autophagy pathway, or interactors beyond autophagy, we performed an interactome analysis through mass spectrometry. This analysis revealed a host of proteins ... More