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Deletion of Asb15b gene can lead to a significant decrease in zebrafish intermuscular bone

Gene. 2024-05; 
Minghui Niu, Huamin Whang, Zhichao Wu, Shouwen Jiang, Liangbiao Chen
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摘要

Intermuscular bones, which are present in numerous economically significant fish species, have a negative impact on the development of aquaculture. The Asb15b gene, primarily expressed in skeletal muscle, plays a crucial role in regulating protein turnover and the development of muscle fibers. It stimulates protein synthesis and controls the differentiation of muscle fibers. In this study, we employed CRISPR/Cas9 technology to generate homozygous zebrafish strains with 7?bp and 49?bp deletions in the Asb15b gene. Subsequent analyses using skeleton staining demonstrated a substantial reduction in the number of intermuscular bones in adult Asb15b -7 bp and Asb15b -49?bp mutants compared to the wild-type zebraf... More

關鍵詞

Asb15b gene, CRISPR/Cas9, Intermuscular bone, Zebrafish
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