產品信息

常見問題解答

• 檢測過程需要用到哪些設備？

  ToxinSensor? Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350 & L00350C) 需要使用可設置 37 ℃ ± 1 ℃ 的水浴鍋進行孵育，使用帶有 545 nm 濾光片的分光光度計或酶標儀進行結果的讀取；ToxinSensor? Gel clot Endotoxin Assay Kit (Cat. No. L00351) 和 ToxinSensor? Single Test Kits (Cat. No.L00856-L00859) 僅需要使用水浴鍋或凝膠測定儀進行孵育即可。

• 能否用烘箱進行孵育？

  建議使用水浴或凝膠測定儀進行孵育，烘箱/搖床可能由于溫度分布不均一導致孵育不充分。

• 哪些類型的樣品與 ToxinSensor? 內毒素檢測系統不兼容？

  金斯瑞內毒素檢測試劑盒不適用于人類疾病診斷、臨床樣本（例如抗生素樣本）、患者管理和細胞/細菌培養基中的內毒素檢測。此外，由于 β-葡聚糖會非特異性干擾鱟試劑并導致假陽性結果，我們不建議使用我們的內毒素檢測試劑盒檢測 β-葡聚糖樣品或受 β-葡聚糖污染的樣品。一般來說，在檢測樣品的內毒素水平之前，建議按照《中國藥典》進行干擾測試。確保消除干擾因素。
  對于血液血清樣品，可以用檢查用水或樣品處理液稀釋10倍，再加熱處理后進行檢測。加熱溫度可以70℃，10min,也可根據樣品進行調整。

• 樣本怎么處理才能進行檢測？

  根據藥典要求，當供試品新建內毒素檢測時須先進行內毒素干擾實驗，或當供試品或鱟試劑的生產工藝或環境發生了可能影響實驗結果的變化時需要進行內毒素干擾試驗后再執行正式的檢測。另外樣本本身的pH對于內毒素檢測準確性十分重要, 因此，如有必要，我們建議使用氫氧化鈉 （0.1 M，溶解在 LAL 試劑水中） 或鹽酸 （0.1 M，溶解在 LAL 試劑水中）調節 pH 值至6-8。

• 配制內毒素標準溶液時可以使用塑料器皿嗎？

  相較于玻璃表面，內毒素更容易黏附在塑料制品表面，金斯瑞提供 ToxinSensor? 無內毒素試管（Cat.No.: M01072）供您稀釋或分裝樣品。

客戶引文

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6. O'Meara CP., et al. Immunization with a MOMP-Based Vaccine Protects Mice against a Pulmonary Chlamydia Challenge and Identifies a Disconnection between Infection and Pathology. PLoS One. 2013 Apr 16;8(4):e61962.
7. Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
8. Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
9. Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
10. Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
11. A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
12. Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
13. Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
14. K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
15. Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
16. Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
17. Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
18. Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
19. Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
20. Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
21. Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
22. Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
23. Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.
24. Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
25. Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
26. Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
27. Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
28. A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
29. Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
30. Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
31. K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
32. Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
33. Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
34. Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
35. Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
36. Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
37. Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
38. Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
39. Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
40. Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.