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BDSF Is a Degradation-Prone Quorum-Sensing Signal Detected by the Histidine Kinase RpfC of pv

Appl Environ Microbiol. 2022-04; 
Xiu-Qi Tian, Yao Wu, Zhen Cai, Wei Qian
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Recombinant Proteins … After the injection of 2 U of PreScission protease (GenScript), the column was sealed and placed on a rotator at 4C. After a 10-h digestion, the flowthrough fractions, which contained the … Get A Quote

摘要

Diffusible signal factors (DSFs) are medium-chain fatty acids that induce bacterial quorum sensing. Among these compounds, BDSF is a structural analog of DSF that is commonly detected in bacterial species, and it is the predominant quorum-sensing signal in Xanthomonas campestris. How BDSF is sensed in spp. and the functional diversity between BDSF and DSF remain unclear. In this study, we generated genetic and biochemical evidence that BDSF is a low-active regulator of X. campestris pv. quorum sensing, whereas -BDSF does not seem to be a signaling compound. BDSF is detected by the sensor histidine kinase RpfC. Although BDSF has relatively low physiological activities, it binds to the RpfC sensor with a high ... More

關鍵詞

BDSF, DSF, Xanthomonas campestris, functional divergence, histidine kinase, quorum-sensing
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