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Resveratrol Protects Optic Nerve Head Astrocytes from Oxidative Stress-Induced Cell Death by Preventing Caspase-3 Activation, Tau Dephosphorylation at Ser422 and Formation of Misfolded Protein Aggregates

Cell Mol Neurobiol. 2020; 
Means JC, Lopez AA, Koulen P,
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Recombinant Proteins Western blots were performed to detect caspase-cleaved Tau, a mouse anti-Tau, caspase-cleaved (truncated at Asp421) antibody (MilliporeSigma, Billerica, MA, USA; product number, MAB5430) was used at a dilution of 1:500. Tau phosphorylated at Ser422 was detected using a rabbit anti-Tau (phospho-Ser422) (GenScript, Piscataway, NJ, USA; product number, A00900) antibody at a dilution of 1:1000. The Tau 5A6 antibody (Developmental Studies Hybridoma Bank, Iowa City, IA, USA; product number, 5A6) was used to detect full length Tau at a dilution of 1:500. GFAP antibody (ab4674, abcam) was used at a concentration of 1:1000. A mouse anti-actin antibody (MilliporeSigma; product number, MAB1501R) was used as a loading control at a dilution of 1:1000. Get A Quote

摘要

Optic nerve head astrocytes (ONHAs) are the major cell type within the optic nerve head, providing both structural and nutrient support to the optic nerve. Astrocytes are necessary for the survival of neurons with controlled activation of astrocytes being beneficial to neurons. However, overactive astrocytes can be harmful and the loss of normal astrocyte function can be a primary contributor to neurodegeneration. The neuroprotective properties of reactive astrocytes can be lost or they might gain neurotoxic properties in neurodegenerative diseases. The activated astrocytes are crucial in the development of glaucoma, where they serve as a source for cytotoxic substances that participate in ganglion apoptosis. T... More

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