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Inducible Plasmid Self-Destruction (IPSD) Assisted Genome Engineering in Lactobacilli and Bifidobacteria.

ACS Synth Biol. 2019; 
ZuoFanglei,ZengZhu,Hammarstr?mLennart,MarcotteHa
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Recombinant Proteins The cultures were inoculated (1%, v/v) into MRS broth without antibiotics and grown at 37 °C until OD600nm reached ~0.30, then supplemented with 100 ng/mL sakacin P (SppIP) (Genscript). Get A Quote

摘要

Genome engineering is essential for application of synthetic biology in probiotics including lactobacilli and bifidobacteria. Several homologous recombination system-based mutagenesis tools have been developed for these bacteria, but still have many limitations in different species or strains. Here we developed a genome engineering method based on an inducible self-destruction plasmid delivering homologous DNA into bacteria. Excision of the replicon by induced recombinase facilitates selection of homologous recombination events. This new genome editing tool called inducible plasmid self-destruction (IPSD) was successfully used to perform gene knockout and knock-in in lactobacilli and bifidobacteria. Due to it... More

關鍵詞

bifidobacteria,genome engineering,homologous recombination,lactobacilli,synthetic bio
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