国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

至今,GenScript的服務及產品已被Cell, Nature, Science, PNAS等1300多家生物醫藥類雜志引用近萬次,處于行業領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發表科研成果,再次證明GenScript 有能力幫助業內科學家Make research easy.

Inducible Plasmid Self-Destruction (IPSD) Assisted Genome Engineering in Lactobacilli and Bifidobacteria.

ACS Synth Biol. 2019; 
ZuoFanglei,ZengZhu,Hammarstr?mLennart,MarcotteHa
Products/Services Used Details Operation
Recombinant Proteins The cultures were inoculated (1%, v/v) into MRS broth without antibiotics and grown at 37 °C until OD600nm reached ~0.30, then supplemented with 100 ng/mL sakacin P (SppIP) (Genscript). Get A Quote

摘要

Genome engineering is essential for application of synthetic biology in probiotics including lactobacilli and bifidobacteria. Several homologous recombination system-based mutagenesis tools have been developed for these bacteria, but still have many limitations in different species or strains. Here we developed a genome engineering method based on an inducible self-destruction plasmid delivering homologous DNA into bacteria. Excision of the replicon by induced recombinase facilitates selection of homologous recombination events. This new genome editing tool called inducible plasmid self-destruction (IPSD) was successfully used to perform gene knockout and knock-in in lactobacilli and bifidobacteria. Due to it... More

關鍵詞

bifidobacteria,genome engineering,homologous recombination,lactobacilli,synthetic bio
              主站蜘蛛池模板: 连江县| 涡阳县| 余江县| 集贤县| 沈丘县| 德庆县| 中江县| 遂溪县| 灌阳县| 大洼县| 云南省| 曲麻莱县| 东阿县| 曲阜市| 林口县| 莱州市| 信丰县| 台山市| 青海省| 许昌市| 五寨县| 龙州县| 布尔津县| 内丘县| 维西| 陆川县| 泊头市| 昌平区| 宿州市| 简阳市| 六安市| 会理县| 岳池县| 驻马店市| 如东县| 峨山| 富民县| 扶风县| 夏津县| 夏河县| 景洪市|