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Nucleic Acid Purification & Analysis> | … The beads were washed five times with a solution containing 20 mM Tris (pH 7.4), 150 mM NaCl, and 0.05% Tween 20; separated on an SDS-PAGE gel; and analyzed by immunoblot using an anti-GST antibody (GenScript; A00866-100, lot 13D000626, Shanghai, China) or an … | Get A Quote |
Seeds germination or dormancy is strictly controlled by endogenous phytohormone signal and environment cues. High temperature (HT) suppresses seeds germination or triggers seeds dormancy but underlying mechanism by which HT mediates seeds germination thermoinhibition needs more investigating. SOM is reported as the critical factor negatively controls light-irradiation seeds germination by altering Abscisic acid (ABA) and gibberellin acid (GA) biosynthesis. Here we found that HT accelerates SOM expressing through ABA signal transduction component ABI3, both of abi3 and som mutants seeds show high germination rate under HT in contrast to wild type seeds. Using ABI3 as the bait, we identified the epigenetic fa... More
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