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Impact of Equilibrative Nucleoside Transporters TgAT1, TgENT1, and TgENT3 on Toxoplasma gondii Infection and Differentiation

biorxiv. 2024-07; 
Amber Goerner, Gabriel Messina, Lea Drogalis Beckham, Bruno Martorelli Di Genova
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Gene Synthesis All final plasmid sequences are listed in the plasmid list (supplemental file). Plasmids were constructed using standard molecular biology techniques according manufacturer’s protocols. ? ΔTgENT3 Donor: Synthesized and ordered from GenScript ? mAID-HA-TgENT1 Donor: Synthesized and ordered from GenScript (plasmid mAID-HA-TgENT1 donor). Get A Quote

摘要

The protozoan parasite Toxoplasma gondii relies on its host for essential metabolites, including purines, which it cannot synthesize de novo. This study investigates the roles of equilibrative nucleoside transporters (TgENTs), specifically TgAT1, TgENT1, and TgENT3, across different developmental stages of T. gondii. Utilizing protein-protein BLAST and position-specific iterated BLAST, we identified six genes in the T. gondii genome with high sequence similarity to human and Plasmodium falciparum ENTs, focusing on TgENT1, TgENT3, and TgAT1 due to their expression in tachyzoites and homology to the nucleoside domain. Our results demonstrate that TgENT3 plays a crucial role during chronic infection, as T... More

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