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miR-193b-5p promotes GCRV replication by inhibiting autophagy via targeting deptor in grass carp (Ctenopharyngodon idellus)

Fish Shellfish Immunol. 2024-02; 
Hongyan Yu, Zheyan Chen, Qiaozhen Yu, Yubang Shen, Lang Gui, Xiaoyan Xu, Jiale Li
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Proteins, Expression, Isolation and Analysis … Proteins from cells were extracted with RIPA Lysis Buffer with … by 4–20% PAGE (GenScript, China), followed by transferring … After all these measures mentioned above, the target protein … Get A Quote

摘要

miRNAs are increasingly recognized for their crucial role in autophagy processes. Recent research has highlighted the significant function of autophagy in modulating immune responses. Within this context, specific miRNAs have been identified as indirect mediators of immune functions through their modulation of autophagy. In this study, we verified that miR-193b-5p simultaneously targeted the grass carp autophagy-related gene deptor, thereby reducing autophagy levels in CIK cells. Moreover, we found the expression levels of miR-193b-5p and deptor responding to pathogen infections in the GCRV-infected CIK cells. Notably, the overexpression of miR-193b-5p was found to induce the GCRV replication and reduce the irf... More

關(guān)鍵詞

Autophagy, GCRV, Grass carp, miR-193b-5p
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