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Evaluating the clinical utility of semi-quantitative luciferase immunosorbent assay using antigens in syphilis diagnosis and treatment monitoring

Emerg Microbes Infect. 2024-05; 
Wujian Ke, Cailing Ao, Ran Wei, Xiaozhen Zhu, Jingwei Shui, Jianhui Zhao, Xiaohui Zhang, Liuyuan Wang, Liping Huang, Xinying Leng, Rui Zhu, Jiaxin Wu, Lixia Huang, Nanxuan Huang, Haiying Wang, Wenjia Weng, Ligang Yang, Shixing Tang
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Proteins, Expression, Isolation and Analysis … To determine the activity and expression level of fusion proteins, … coated with 5 μg/mL of protein G (Genscript Co., China) in 0.01 M … fusion proteins. (c) A white microtiter plate coated with … Get A Quote

摘要

To assess the clinical applicability of a semi-quantitative luciferase immunosorbent assay (LISA) for detecting antibodies against antigens TP0171 (TP15), TP0435 (TP17), and TP0574 (TP47) in diagnosing and monitoring syphilis. LISA for detection of anti-TP15, TP17, and TP47 antibodies were developed and evaluated for syphilis diagnosis using 261 serum samples (161 syphilis, 100 non-syphilis). Ninety serial serum samples from 6 syphilis rabbit models (3 treated, 3 untreated) and 110 paired serum samples from 55 syphilis patients were used to assess treatment effects by utilizing TRUST as a reference. Compared to TPPA, LISA-TP15, LISA-TP17, and LISA-TP47 showed a sensitivity of 91.9%, 96.9%, and 98.8%, specifici... More

關鍵詞

TP0171 (TP15), TP0435 (TP17), TP0574 (TP47), Treponema pallidum, luciferase immunosorbent assay (LISA), syphilis diagnosis, treatment monitoring, treponemal antibodies
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