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Production and Immunogenicity Assessment of LTp50: An -Made Chimeric Antigen Targeting S1- and S2-Epitopes from the SARS-CoV-2/BA5 Spike Protein

Pharmaceuticals (Basel). 2024-02; 
Alejandra Wong-Arce, Omar Gonzalez-Ortega, Andrea Romero-Maldonado, Arleth Miranda-López, Mariano García-Soto, Susan Farfán-Castro, Lourdes Betancourt-Mendiola, Samaporn Teeravechyan, Kanjana Srisutthisamphan, Mauricio Comas-García, Karla I Solís Andrade, Sergio Rosales-Mendoza
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Proteins, Expression, Isolation and Analysis … RNAs, proteins, and rAAV viral particles. We identified the expression of cap proteins as one of … The plasmid, pCMV-E1, and pCMV-Cap were synthesized at GenScript. Plasmids were … Get A Quote

摘要

Subunit vaccines stand as a leading approach to expanding the current portfolio of vaccines to fight against COVID-19, seeking not only to lower costs but to achieve long-term immunity against variants of concern and have the main attributes that could overcome the limitations of the current vaccines. Herein a chimeric protein targeting S1 and S2 epitopes, called LTp50, was designed as a convenient approach to induce humoral responses against SARS-CoV-2. LTp50 was produced in recombinant using a conventional pET vector, recovering the expected antigen in the insoluble fraction. LTp50 was purified by chromatography (purity > 90%). The solubilization and refolding stages helped to obtain a stable protein amenabl... More

關鍵詞

COVID-19, built-in adjuvant, chimeric antigen, humoral response, linear epitopes
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