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Recombinant Art v401 protein produces immunological tolerance by subcutaneous immunotherapy in a wormwood pollen-driven allergic asthma female mouse model

PLoS ONE. 2024-06; 
Tao Wang, Jiaoni Chi, Zhimin Li, Yue Zhang, Yaojun Wang, Ming Ding, Bin Zhou, JiaChen Gui, Qiang Li
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Molecular Biology Reagents … pollen protein concentration, and the protein samples were analyzed by SDS-PAGE. The LPS level was detected at a concentration of >10 EU/mg (Genscript Toxinsensor kit, L00350). … Get A Quote

摘要

Art v4.01 is a well-known profilin protein belonging to the pan-allergens group and is commonly involved in triggering allergic asthma, polyallergy, and cross-sensitization. It is also referred to as Wormwood due to its origin. Crude wormwood extracts are applied for allergen-specific immunotherapy (AIT). Whether the recombinant Art v4.01 (rArt v4.01) can produce in vivo immunological tolerance by subcutaneous immunotherapy (SCIT) remains elusive. In this study, to investigate the in vivo immunological response of rArt v4.01, Th2, Th1, Treg, Th17 type-related cytokines and phenotypes of immune cells were tested, facilitating the exploration of the underlying mechanisms. The expression and purification of Art v4... More

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