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Saccharomyces cerevisiae cellular engineering for the production of FAME biodiesel

AMB Express. 2024-04; 
Laiyou Wang, Bingbing Liu, Qingshan Meng, Chunchun Yang, Yiyi Hu, Chunyan Wang, Pengyu Wu, Chen Ruan, Wenhuan Li, Shuang Cheng, Shuxian Guo
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Gene Synthesis … cerevisiae genomic DNA was used to amplify the TPI1p promoter, while the codon-optimized DmJHAMT gene fragments were synthesized by GenScript (Nanjing, China). The … Get A Quote

摘要

The unsustainable and widespread utilization of fossil fuels continues to drive the rapid depletion of global supplies. Biodiesel has emerged as one of the most promising alternatives to conventional diesel, leading to growing research interest in its production. Microbes can facilitate the de novo synthesis of a type of biodiesel in the form of fatty acid methyl esters (FAMEs). In this study, Saccharomyces cerevisiae metabolic activity was engineered to facilitate enhanced FAME production. Initially, free fatty acid concentrations were increased by deleting two acetyl-CoA synthetase genes (FAA1, FAA4) and an acyl-CoA oxidase gene (POX1). Intracellular S-adenosylmethionine (SAM) levels were then enhanced via th... More

關鍵詞

Saccharomyces cerevisiae, Biodiesel, Fatty acid methyl esters, Free fatty acids, S-adenosylmethionine
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