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Long-term stability and immunogenicity of lipid nanoparticle COVID-19 mRNA vaccine is affected by particle size

Hum Vaccin Immunother. 2024-05; 
Ruimeng Shi, Xueli Liu, Yajuan Wang, Meilu Pan, Shaoqin Wang, Lin Shi, Beibei Ni
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Proteins, Expression, Isolation and Analysis … For ELISA, a 96-well ELISA microplate was coated with 2 ng/μL of S protein (GenScript) in coating buffer (1×phosphate buffered saline) and incubated overnight at 4C. The wells were … Get A Quote

摘要

Messenger ribonucleic acid (mRNA) technology has been rapidly applied for the development of the COVID-19 vaccine. However, naked mRNA itself is inherently unstable. Lipid nanoparticles (LNPs) protect mRNAs from extracellular ribonucleases and facilitate mRNA trafficking. For mRNA vaccines, antigen-presenting cells utilize LNPs through uptake to elicit antigen-specific immunity. There are reports on the impact of various physical characteristics of LNPs, particularly those with sizes less than 200?nm, especially 50 to 150?nm, on the overall stability and protective efficacy of mRNA vaccines. To address this, a single change in the size of LNPs using the same mRNA stock solution was assessed for the physicoc... More

關鍵詞

SARS-CoV-2, biological activity, lipid nanoparticle, mRNA vaccine, particle size, stability
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