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An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv Chachiensis

Nat Commun. 2024-05; 
Jiawen Wen, Yayu Wang, Xu Lu, Huimin Pan, Dian Jin, Jialing Wen, Canzhi Jin, Sunil Kumar Sahu, Jianmu Su, Xinyue Luo, Xiaohuan Jin, Jiao Zhao, Hong Wu, E-Hu Liu, Huan Liu
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Recombinant Proteins … The gene was sequenced by GenScript Ltd (Nanjing, China). The recombinant plasmid pET28a (+)-CcOMT1 was transformed into E. coli BL21(DE3) (Weidi Biotech, China) for … Get A Quote

摘要

Citrus reticulata cv. Chachiensis (CRC) is an important medicinal plant, its dried mature peels named "Guangchenpi", has been used as a traditional Chinese medicine to treat cough, indigestion, and lung diseases for several hundred years. However, the biosynthesis of the crucial natural products polymethoxylated flavonoids (PMFs) in CRC remains unclear. Here, we report a chromosome-scale genome assembly of CRC with the size of 314.96?Mb and a contig N50 of 16.22?Mb. Using multi-omics resources, we discover a putative caffeic acid O-methyltransferase (CcOMT1) that can transfer a methyl group to the 3-hydroxyl of natsudaidain to form 3,5,6,7,8,3',4'-heptamethoxyflavone (HPMF). Based on transient overexpressio... More

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