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Nanolevel of detection of ascorbic acid using horse-radish peroxidase inhibition assay

Heliyon. 2024-05; 
Bijon Kumar Sil, Mohd Raeed Jamiruddin, Md Ahsanul Haq, Nattanit Aekwattanaphol, Prem Ananth K, Limbadri Salendra, Himanshu Paliwal, Pijush Kumar Paul, Wilaiporn Buatong, Teerapol Srichana
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Proteins, Expression, Isolation and Analysis … The purity of HRP was determined using Genscript SurePage? (GenDScript Biotech, USA) protocol against reference HRP (Cat. N0. P8375-2KU, Sigma-Aldrich, USA) while the band … Get A Quote

摘要

Ascorbic acid plays a significant role in regulation of various bodily functions with high concentrations in immune cells and being involved in connective tissue maintenance. Commonly it is detected through various colorimetric methods. In this study, we propose a one-step simple method based on the inhibitory activity of ascorbic acid on horseradish peroxidase and hydrogen peroxide. The detection is observed by colorimetric changes to TMB (3,3',5,5' tetramethylbenzidine). The enzyme inhibition unit was optimized with a high level of linearity (r?=?0.9999) and the level of detection and level of quantification were found to be 1.35?nM and 4.08?nM, respectively with higher sensitive compared to the HPLC meth... More

關鍵詞

Ascorbic acid, Enzyme inhibition assay, Horse radish peroxidase, Limit of detection, Nanomolar, Percent inhibition
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