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Quantitation of Enterovirus A71 Empty and Full Particles by Sedimentation Velocity Analytical Ultracentrifugation

Viruses. 2024-04; 
Anna Yang, Yun Luo, Jie Yang, Tingbo Xie, Wenhui Wang, Xin Wan, Kaiwen Wang, Deqin Pang, Dongsheng Yang, Hanyu Dai, Jie Wu, Shengli Meng, Jing Guo, Zejun Wang, Shuo Shen
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Proteins, Expression, Isolation and Analysis … The virus proteins were separated on a 4–20% gradient polyacrylamide gel (Genscript, SurePAGE?, Nanjing, China) at 80 mA for an hour and a half. Carbon-coated copper grids (200 … Get A Quote

摘要

The enterovirus A71 (EV71) inactivated vaccine is an effective intervention to control the spread of the virus and prevent EV71-associated hand, foot, and mouth disease (HFMD). It is widely administered to infants and children in China. The empty particles (EPs) and full particles (FPs) generated during production have different antigenic and immunogenic properties. However, the antigen detection methods currently used were established without considering the differences in antigenicity between EPs and FPs. There is also a lack of other effective analytical methods for detecting the different particle forms, which hinders the consistency between batches of products. In this study, we analyzed the application of... More

關鍵詞

EV71 inactivated vaccine, SV-AUC, empty particles, full particles, quality control
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