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Development of an efficient method for selection of stable cell pools for protein expression and surface display with Expi293F cells

Cell Biochem Funct. 2023-03; 
Chuan Chen, Zening Wang, Zehua Sun, Wei Li, Dimiter S Dimitrov
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Recombinant Proteins … Culture medium was clarified by centrifugation, RBD-Fc and KTN0929-IgG were purified by protein A affinity chromatography (GenScript) following the manufacturer's protocol. PRTG-… Get A Quote

摘要

Compare with transient expression, stable cell lines generally have higher productivity and better quality for protein expression. However, selection of stable cell line is time-consuming and laborious. Here we describe an optimized selection method to achieve high-efficient stable cell pools with Expi293F suspension cells. This method only takes 2-3 weeks to generate stable cell pools with 2- to 10-fold higher productivity than transient gene expression (TGE). In fed-batch culture with Yeastolate, >1?g/L yield was achieved with our KTN0239-IgG stable cell pool in shaker flasks. This method can be also applied to efficiently display proteins on the cell surface.

關鍵詞

Bicistronic plasmid, Expi293F, antibody, fed-bath expression, stable cell pool, surface display, transient gene expression
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