国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

至今,GenScript的服務及產(chǎn)品已被Cell, Nature, Science, PNAS等1300多家生物醫(yī)藥類雜志引用近萬次,處于行業(yè)領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發(fā)表科研成果,再次證明GenScript 有能力幫助業(yè)內(nèi)科學家Make research easy.

Development of an efficient method for selection of stable cell pools for protein expression and surface display with Expi293F cells

Cell Biochem Funct. 2023-03; 
Chuan Chen, Zening Wang, Zehua Sun, Wei Li, Dimiter S Dimitrov
Products/Services Used Details Operation
Recombinant Proteins … Culture medium was clarified by centrifugation, RBD-Fc and KTN0929-IgG were purified by protein A affinity chromatography (GenScript) following the manufacturer's protocol. PRTG-… Get A Quote

摘要

Compare with transient expression, stable cell lines generally have higher productivity and better quality for protein expression. However, selection of stable cell line is time-consuming and laborious. Here we describe an optimized selection method to achieve high-efficient stable cell pools with Expi293F suspension cells. This method only takes 2-3 weeks to generate stable cell pools with 2- to 10-fold higher productivity than transient gene expression (TGE). In fed-batch culture with Yeastolate, >1?g/L yield was achieved with our KTN0239-IgG stable cell pool in shaker flasks. This method can be also applied to efficiently display proteins on the cell surface.

關鍵詞

Bicistronic plasmid, Expi293F, antibody, fed-bath expression, stable cell pool, surface display, transient gene expression
              主站蜘蛛池模板: 天水市| 赫章县| 天柱县| 永昌县| 潞城市| 嘉义县| 巨野县| 临清市| 渭源县| 攀枝花市| 马尔康县| 石嘴山市| 土默特左旗| 康马县| 美姑县| 赫章县| 洞口县| 石楼县| 方城县| 浠水县| 内江市| 吉木乃县| 道孚县| 额济纳旗| 新郑市| 永城市| 临高县| 石狮市| 郎溪县| 阳谷县| 磐安县| 泽州县| 奉新县| 南城县| 蚌埠市| 马公市| 威海市| 临西县| 公安县| 浙江省| 凤山市|