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A semi-quantitative upconversion nanoparticle-based immunochromatographic assay for SARS-CoV-2 antigen detection

Front Microbiol. 2023-12; 
Hai Ding, Wanying Zhang, Shu-An Wang, Chuang Li, Wanting Li, Jing Liu, Fang Yu, Yanru Tao, Siyun Cheng, Hui Xie, Yuxin Chen
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Proteins, Expression, Isolation and Analysis … the power of science and technology advancement in responding to a major infectious … This assay has been commercialized under the tradename cPassTM by GenScript Biotech, … Get A Quote

摘要

The unprecedented public health and economic impact of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been met with an equally unprecedented scientific response. Sensitive point-of-care methods to detect SARS-CoV-2 antigens in clinical specimens are urgently required for the rapid screening of individuals with viral infection. Here, we developed an upconversion nanoparticle-based lateral flow immunochromatographic assay (UCNP-LFIA) for the high-sensitivity detection of SARS-CoV-2 nucleocapsid (N) protein. A pair of rabbit SARS-CoV-2?N-specific monoclonal antibodies was conjugated to UCNPs, and the prepared UCNPs were then d... More

關(guān)鍵詞

SARS-CoV-2, antigen detection, fluorescence immunochromatography assay, semi-quantitative, upconversion nanoparticles
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