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In silico design, production and immunization evaluation of a recombinant bivalent fusion protein candidate vaccine against E coli O157:H7

Int Immunopharmacol. 2022-11; 
Hossein Samiei, Shahram Nazarian, Abass Hajizade, Emad Kordbacheh
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Proteins, Expression, Isolation and Analysis … performed using GenScript service. Theoretical analysis of DNA parameter optimization was done by using Rare Codon Calculator tool of GenScript web page (https://www.genscript.… Get A Quote

摘要

In silico techniques are highly suited for both the discovery of new and development of available vaccines. Escherichia coli O157: H7, a main cause of food poisoning can infect humans through the consumption of contaminated water or food. Vaccination is a choice strategy to combat the bacterium. In the present study, we designed, expressed and purified a chimeric protein comprising two antigens of Escherichia coli O157: H7, including intimin and flagellin proteins, as a vaccine candidate and evaluated its immunization ability in mice. Thein silicoresults showed that the proposed antigen has a high antigenicity and conformation to be used as a potent vaccine candidate. The protein was successfully expressed in E... More

關(guān)鍵詞

Bioinformatics design, Chimer gene, E. coli O157: H7, EHEC, Intimin
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