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Differential activation of mouse and human Panx1 channel variants

PLoS ONE. 2023-12; 
Antonio Cibelli, Preeti Dohare, David C Spray, Eliana Scemes
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Proteins, Expression, Isolation and Analysis … in the pEGC Bacmam vector from our previous study was used(14). Mouse PANX1 (UniprotID: Q9JIP4) was synthesized by GenScript and subcloned into the pEGC Bacmam vector(41). … Get A Quote

摘要

Pannexins are ubiquitously expressed in human and mouse tissues. Pannexin 1 (Panx1), the most thoroughly characterized member of this family, forms plasmalemmal membrane channels permeable to relatively large molecules, such as ATP. Although human and mouse Panx1 amino acid sequences are conserved in the presently known regulatory sites involved in trafficking and modulation of the channel, differences are reported in the N- and C-termini of the protein, and the mechanisms of channel activation by different stimuli remain controversial. Here we used a neuroblastoma cell line to study the activation properties of endogenous mPanx1 and exogenously expressed hPanx1. Dye uptake and electrophysiological recordings r... More

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