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Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity

Cell Research . 2023-11;

Le Zheng, Tingting Yang , Hangtian Guo , Chen Qi , Yuchi Lu , Haonan Xiao , Yan Gao , Yue Liu, Yixuan Yang , Mengru Zhou , Henry C Nguyen 1, Yun Zhu 8, Fei Sun 9 10, Chen-Yu Zhang , Xiaoyun Ji

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Codon Optimization	The full-length cDNAs for human SIDT1 and SIDT2 (Uniport: Q9NXL6 and Q8NBJ9, respectively) were codon-optimized and synthesized by GenScript Co., Ltd./The extraction was centrifuged at 39,000 × g (Beckman) for 60 minutes to remove insoluble components, and the supernatant was incubated with anti-Flag affinity resin (GenScript Co., Ltd.) at 4 °C for 90 minutes.	Get A Quote
Peptide Synthesis		Get A Quote