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Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production

Food Research International. 2023-05; 
Qingzi Lei, Jun Ma, Guocheng Du, Jingwen Zhou, Xin Guan
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Proteins, Expression, Isolation and Analysis Commercial bFGF, EGF, LR3 -IGF-1, and PDGF-BB were purchased from GenScript Corporation (Nanjing, China) Get A Quote

摘要

Cultured meat technology is a novel and promising alternative strategy for meat production, and it provides an efficient, safe, and sustainable way to supply animal protein. Cytokines play an important role in promoting the rapid proliferation of cells, but the high cost and potential food safety concerns of commercial cytokines have hindered their application in large-scale cultured meat production. Herein, Saccharomyces cerevisiae C800 was used as a starting strain in which four cytokines were exogenously expressed simultaneously using the Cre-loxP system, including long-chain human insulin growth factor-1, platelet-derived growth factor-BB, basic fibroblast growth factor, and epidermal growth fac... More

關鍵詞

Cultured meat Cytokine combination Co-expression Muscle satellite cells Saccharomyces cerevisiae
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