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Immunological characterization of chicken tumor necrosis factor-α (TNF-α) using new sets of monoclonal antibodies specific for poultry TNF-α

Dev Comp Immunol. 2022-02; 
Mingmin Lu, Youngsub Lee, Charles Li, Hyun S Lillehoj
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Proteins, Expression, Isolation and Analysis … The protein rchTNF-α was used for the immunization of mice. Immunization, fusion, and selection for the generation of hybridomas were conducted and processed by GenScript Inc?… Get A Quote

摘要

Tumor necrosis factor-α (TNF-α) is a type II transmembrane protein with either membrane-bound or soluble forms and is a prototypical member of the TNF superfamily. TNF-α is a pleiotropic cytokine associated with the regulation of systemic inflammation and host defense. Chicken TNF-α (chTNF-α) is a long-missed avian ortholog, and its immunological properties remain largely unknown compared to those of its mammalian counterparts. Here, we report the functional characterization and immunomodulatory properties of chTNF-α using a panel of newly developed anti-chTNF-α mouse monoclonal antibodies (mAbs). Using anti-chTNF-α mAbs, we determined the tissue expression of chTNF-α in lymphoid and non-lymphoid organ... More

關鍵詞

Capture assay, Chicken, Intracellular expression, Monoclonal antibody, Nitric oxide, TNF-α
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