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Activating the Cpx response induces tolerance to antisense PNA delivered by an arginine-rich peptide in

Mol Ther Nucleic Acids. 2021-06; 
Jakob Frimodt-M?ller, Andreas Koulouktsis, Godefroid Charbon, Marit Otterlei, Peter E Nielsen, Anders L?bner-Olesen
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摘要

Cell-penetrating peptides (CPPs) are increasingly used for cellular drug delivery in both pro- and eukaryotic cells, and oligoarginines have attracted special attention. How arginine-rich CPPs translocate across the cell envelope, particularly for prokaryotes, is still unknown. Arginine-rich CPPs efficiently deliver antimicrobial peptide nucleic acid (PNA) to its intracellular mRNA target in bacteria. We show that resistance to PNA conjugated to an arginine-rich CPP in requires multiple genetic modifications and is specific for the CPP part and not to the PNA part. An integral part of the resistance was the constitutively activated Cpx-envelope stress response system (), which decreased the cytoplasmic membran... More

關鍵詞

Cpx response, aminoglycosides, arginine-rich cell-penetrating peptides, inner membrane, peptide nucleic acids
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