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Fabrication of MERS-nanovesicle biosensor composed of multi-functional DNA aptamer/graphene-MoS nanocomposite based on electrochemical and surface-enhanced Raman spectroscopy

Sens Actuators B Chem. 2021-11; 
Gahyeon Kim, Jinmyeong Kim, Soo Min Kim, Tatsuya Kato, Jinho Yoon, Seungwoo Noh, Enoch Y Park, Chulhwan Park, Taek Lee, Jeong-Woo Choi
Products/Services Used Details Operation
Recombinant Proteins and thiourea was obtained from Tokyo Chemical Industry Co., Ltd., (Tokyo, Japan). Graphene oxide (GO) was purchased from Graphene Supermarket (NY, USA). Streptavidin MagBeads was purchased from GenScript (NJ, USA) Get A Quote

摘要

Middle East respiratory syndrome coronavirus (MERS-CoV) is one of the most harmful viruses for humans in nowadays. To prevent the spread of MERS-CoV, a valid detection method is highly needed. For the first time, a MERS-nanovesicle (NV) biosensor composed of multi-functional DNA aptamer and graphene oxide encapsulated molybdenum disulfide (GO-MoS) hybrid nanocomposite was fabricated based on electrochemical (EC) and surface-enhanced Raman spectroscopy (SERS) techniques. The MERS-NV aptamer was designed for specifically binding to the spike protein on MERS-NVs and it is prepared using the systematic evolution of ligands by exponential enrichment (SELEX) technique. For constructing a multi-functional MERS aptamer... More

關(guān)鍵詞

Aptamer, MERS-NV, MERS-NV biosensor, SELEX, Surface-enhanced Raman spectroscopy
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