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Diacylglycerol kinase A is essential for polymyxin resistance provided by EptA, MCR-1 and other lipid A phosphoethanolamine transferases

J Bacteriol. 2021-11; 
Alexandria B Purcell, Bradley J Voss, M Stephen Trent
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Proteins, Expression, Isolation and Analysis Immunodetection was performed using 1:10,000 dilution of anti-His MAb mouse antibody (Genscript) as the primary antibody and a 1:10,000 dilution of Cy5 goat anti-mouse IgG (Invitrogen) as the secondary antibody.? Get A Quote

摘要

Gram-negative bacteria utilize glycerophospholipids (GPLs) as phospho-form donors to modify various surface structures. These modifications play important roles in bacterial fitness in diverse environments influencing cell motility, recognition by the host during infection, and antimicrobial resistance. A well-known example is the modification of the lipid A component of lipopolysaccharide by the phosphoethanolamine (pEtN) transferase EptA that utilizes phosphatidyethanoalmine (PE) as the phospho-form donor. Addition of pEtN to lipid A promotes resistance to cationic antimicrobial peptides (CAMPs), including the polymyxin antibiotics like colistin. A consequence of pEtN modification is the production of diacylg... More

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