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Armet from whitefly saliva acts as an effector to suppress plant defences by targeting tobacco cystatin

New Phytol. 2022-04; 
Hui Du, Hong-Xing Xu, Fang Wang, Li-Xin Qian, Shu-Sheng Liu, Xiao-Wei Wang
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Proteins, Expression, Isolation and Analysis … BtArmet polyclonal antibody was prepared by GenScript Co. (Nanjing, China) through expressing complete affinity-purified peptide (sequence: CRIEELKPKYVRDEL). To detect BtArmet … Get A Quote

摘要

Arginine rich, mutated in early stage of tumours (Armet), is a well-characterized bifunctional protein as an unfolded protein response component intracellularly and a neurotrophic factor extracellularly in mammals. Recently, a new role of Armet as an effector protein mediating insect-plant interactions has been reported; however, its molecular mechanisms underlying the regulation of plant defences remain unclear. We investigated the molecular mechanisms underlying whitefly-secreted Armet-mediated regulation of insect-plant interaction by agrobacterium-mediated transient expression, RNA interference, electrical penetration graph, protein-protein interaction studies, virus-induced gene silencing assay, phytohormo... More

關鍵詞

Nicotiana tabacum, Armet, cystatin, effector, saliva, tobacco, whitefly, whitefly-plant interactions
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