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Molecular cloning and characterization of a novel bifunctional cellobiohydrolase/β-xylosidase from a metagenomic library of mangrove soil

Enzyme Microb Technol. 2022-10; 
Yi-De Liu, Ge Yuan, Yu-Ting An, Zi-Ran Zhu, Gang Li
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Recombinant Proteins … kit was purchased from GenScript (Piscataway, New Jersey, … Then, 200 μl of 1 M Na 2 CO 3 was added to stop the reaction… Then, 200 μl of 1 M Na 2 CO 3 was added to stop the reaction… Get A Quote

摘要

A metagenomic library of mangrove soil samples consisting of approximately 11,000 clones was constructed, and a rare bifunctional cellobiohydrolase/β-xylosidase Cbh2124 was identified by functional screening. Cbh2124 displayed the highest homology (56.43%) with a protein of the glycoside hydrolase 10 (GH10) family from Proteobacteria. Phylogenetic analysis confirmed that Cbh2124 belongs to the GH10 family. The recombinant enzyme showed a strong cellobiohydrolase activity and a relatively high β-xylosidase activity, and its catalytic efficiency to the cellobiose substrate was as high as 1.27?×?10 s·mM, the highest efficiency among reported cellobiohydrolases. Of particular interest, some enzymatic properti... More

關鍵詞

Bifunctional enzyme, Cellobiohydrolase, Mangroves, Metagenome, β-xylosidase
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