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Genetically engineered pH-responsive silk sericin nanospheres with efficient therapeutic effect on ulcerative colitis

Acta Biomater. 2022-03; 
Sheng Xu, Qianqian Yang, Riyuan Wang, Chi Tian, Yanting Ji, Huanhuan Tan, Ping Zhao, David L Kaplan, Feng Wang, Qingyou Xia
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Proteins, Expression, Isolation and Analysis … Subsequently, 30 μL of each aqueous WT and rhLF sericin solution samples were analyzed on 4-20% SDS-PAGE gels (Genscript, Nanjing, China) and stained by the coomassion … Get A Quote

摘要

Ulcerative colitis (UC) is one type of inflammatory bowel disease (IBD) and lactoferrin (LF) is a promising protein drug to treat UC. However, targeted LF delivery to optimize bioavailability, targeting and effectiveness remains a challenge. Here, we report?an effective strategy to fabricate silk sericin nanospheres systems for the delivery of recombinant human lactoferrin (SS-NS-rhLF). The system is based on the use of optimized transgenic silkworms to generate genetically engineered silk fibers (rhLF-silks). The rhLF silks were used for fabricating SS-NS-rhLF by ethanol precipitation. The SS-NS-rhLF were stable with a spherical morphology with an average diameter of 123 nm. The negatively charged sericins in... More

關鍵詞

Genetical engineering, Lactoferrin, Nanospheres, Sericin, Ulcerative colitis
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