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The development of a candidate of desensitization vaccines against Der f 2 nearly without IgE-binding activity

Immunobiology. 2022-04; 
Xiao-Jing Ling, Chen Pan, Ling-Xiao Tan, Ying Zhu, Yu-Xing Yang, Xiao-Fei Zeng, Jin-Lyu Sun, Ji-Fu Wei, Chen Lu
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Proteins, Expression, Isolation and Analysis … β-D-thiogalactoside at final concentration of 1 mM, and were purified by high-affinity Ni 2+ NTA resin (Genscript, Nanjing, China). Then, purified rWt-Der f 2 and rMt-Der f 2 proteins were … Get A Quote

摘要

Considering the important role of Der f 2 in house dust mites mediating allergic diseases and allergic adverse effects during allergen-specific immunotherapy (AIT), we intend to develop a candidate of desensitization vaccines against Der f 2 without allergenicity. According to the reported immunoglobulin E (IgE)-binding B and T cell epitopes of Der f 2, four candidates of desensitization vaccines against Der f 2 were developed. Recombinant wild-type Der f 2 (rWt-Der f 2) preserved conformational and linear IgE-binding B epitopes. rWt-Der f 2 linearized by reduction and alkylation reactions (rWt-Der f 2 (red/alk)) and recombinant modified-type Der f 2 (rMt-Der f 2) were developed via destroying conformational an... More

關鍵詞

Conformational IgE-binding B epitopes, Der f 2, Desensitization vaccines, Linear IgE-binding B epitopes
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