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miR-145-5p inhibits the proliferation of glioma stem cells by targeting translationally controlled tumor protein

J Cancer. 2022-02; 
Qiujian Zhang, Zhe Cheng, Lei Shi, Gengsheng Mao
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Proteins, Expression, Isolation and Analysis … The proteins were separated using SDS-PAGE (GenScript, Nanjing, China) at 140 V for 50 min. After that, the proteins were transferred onto a PVDF membrane (GenScript) at 300 mA … Get A Quote

摘要

Glioma stem cells (GSCs) have potential for proliferation, self-renewal, and differentiation-the properties that play decisive roles in the process of malignancy in glioma. MicroRNAs (miRNAs) have been shown to regulate the characteristics of cancer stem cells. In this study, we show that miR-145-5p, a recently discovered miRNA, is expressed at low levels in primary GSCs (pGSCs). Upregulation of miR-145-5p resulted in the inhibition of proliferation and increased apoptosis of pGSCs. Furthermore, its overexpression resulted in reduced expression of translationally controlled tumor protein (TCTP). Bioinformatics analysis and luciferase targeting assay revealed that miR-145-5p exerts its effects by directly target... More

關鍵詞

apoptosis, glioma stem cells, miR-145-5p, miRNAs, proliferation, translationally controlled tumor protein
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