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Synthesis of broad-specificity activity-based probes for -β-mannosidases

Org Biomol Chem. 2022-01; 
Nicholas G S McGregor, Chi-Lin Kuo, Thomas J M Beenakker, Chun-Sing Wong, Wendy A Offen, Zachary Armstrong, Bogdan I Florea, Jeroen D C Codée, Herman S Overkleeft, Johannes M F G Aerts, Gideon J Davies
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Proteins, Expression, Isolation and Analysis … buffer and flash frozen with liquid nitrogen until use. Protein concentrations were determined … pET24a between the NdeI and XhoI restriction sites by Genscript (Netherlands) to add a C-… Get A Quote

摘要

-β-mannosidases are a broad class of stereochemically retaining hydrolases that are essential for the breakdown of complex carbohydrate substrates found in all kingdoms of life. Yet the detection of -β-mannosidases in complex biological samples remains challenging, necessitating the development of new methodologies. Cyclophellitol and its analogues selectively label the catalytic nucleophiles of retaining glycoside hydrolases, making them valuable tool compounds. Furthermore, cyclophellitol can be readily redesigned to enable the incorporation of a detection tag, generating activity-based probes (ABPs) that can be used to detect and identify specific glycosidases in complex biological samples. Towards the dev... More

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