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miR-145-5p attenuates inflammatory response and apoptosis in myocardial ischemia-reperfusion injury by inhibiting NOH-1

Exp Anim. 2021-03; 
Lili Tan, Limin Liu, Jian Yao, Chenghao Piao
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Recombinant Proteins … The sequences of all the PCR primers 144 (GenScript, Nanjing, China) were as follows: 5'- GTCCAGTTTTCCCAGGAATCC 145 -3' (forward) and 5'- GCAGGGTCCGAGGTATTC -3' (reverse) for miR-145-5p, 5'- 146 GATCTCGGAAGCTAAGCAGG -3' (forward) and 5'- 147?… Get A Quote

摘要

Myocardial ischemia-reperfusion (I/R) injury is a common complication following reperfusion therapy that involves a series of immune or apoptotic reactions. Studies have revealed the potential roles of miRNAs in I/R injury. Herein, we established a myocardial I/R model in rats and a hypoxia/reoxygenation (H/R) model in H9c2 cells and investigated the effect of miR-145-5p on myocardial I/R injury. After 3 h or 24 h of reperfusion, LVESP, EF, and FS were obviously decreased, and LVEDP was increased. Meanwhile, I/R induced an increase in myocardial infarction area. Moreover, a decrease in miR-145-5p and increase in NOH-1 were observed following I/R injury. With this in mind, we performed a luciferase reporter assa... More

關鍵詞

NOH-1, apoptosis, inflammatory response, miR-145-5p, myocardial ischemia-reperfusion injury
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