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Development of a New Reverse Genetics System for Ebola Virus

mSphere. 2021-05; 
Tianyu Gan, Dihan Zhou, Yi Huang, Shuqi Xiao, Ziyue Ma, Xiaoyou Hu, Yimin Tong, Huimin Yan, Jin Zhong
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Recombinant Proteins GenScript?… Development Program of China (2016YFC1201000) and Advanced Customer Cultivation Project of Wuhan National Biosafety Laboratory, Chinese Academy of?… WG, Duprex WP, Dye JM, Easton AJ, Fooks AR, Formenty PBH, Fouchier RAM, Freitas-Astúa J, Ghedin E?… Get A Quote

摘要

Ebola virus (EBOV) is a highly pathogenic negative-stranded RNA virus that has caused several deadly endemics in the past decades. EBOV reverse genetics systems are available for studying live viruses under biosafety level 4 (BSL-4) or subviral particles under BSL-2 conditions. However, these systems all require cotransfection of multiple plasmids expressing viral genome and viral proteins essential for EBOV replication, which is technically challenging and unable to naturally mimic virus propagation using the subviral particle. Here, we established a new EBOV reverse genetics system only requiring transfection of a single viral RNA genome into an engineered cell line that stably expresses viral nucleoprotein (... More

關(guān)鍵詞

Ebola virus, filovirus, negative-stranded RNA virus, reverse genetics
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