資源 » 參考數據庫 » 客戶發表文獻

至今，GenScript的服務及產品已被Cell, Nature, Science, PNAS等1300多家生物醫藥類雜志引用近萬次，處于行業領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發表科研成果，再次證明GenScript 有能力幫助業內科學家Make research easy.

In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples

STAR Protoc. 2021-09;

Fabien Labroussaa, Kemal Mehinagic, Valentina Cippa, Matthias Liniger, Hatice Akarsu, Nicolas Ruggli, Joerg Jores

Products/Services Used	Details	Operation
Recombinant Proteins	… used in this protocol involves the fragmentation of the ASFV genome into seven sub-genomic fragments as presented in Figure 1. 1. The 5′ and 3′-ends of the ASFV genome, consisting of inverted-terminal-repeats (ITRs), are chemically-synthetized (GenScript) in order to?…	Get A Quote

摘要

This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. In addition, the precise deletion of a non-essential gene and its replacement by a synthetic reporter gene cassette are presented. This protocol is applicable to other ASFV genotypes and other large DNA viruses.