| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … can enhance the response to brain injury through the regulation by some molecules, such as?… HIF-1α siRNA was synthesized by Genscript Biotech Corporation (Nanjing, China?… with radioimmunoprecipitation assay buffer and a Bicinchoninic acid protein assay kit (Bio-Rad, State?… | Get A Quote |
objective: To assess the potential effect of dl-3-N-butylphthalide (dl-NBP) for the proliferation and differentiation of neural stem cells (NSCs) against hypoxia and the underlying mechanism. methods: Hippocampal NSCs were obtained from fetal rats. NSCs combined with dl-NBP and single NSCs were cultured. The impact of siRNA-mediated hypoxia-inducible factor-1alpha (HIF-1α) knockdown on NSCs was detected with western blotting (WB) and quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR). Cell-counting kit-8 assay was used for evaluating the viability of NSCs. Levels of HIF-1α protein were measured using WB, and vascular endothelial growth factor (VEGF) expression was quantified usin... More
