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Chromium induced neurotoxicity by altering metabolism in zebrafish larvae

Ecotoxicol Environ Saf. 2021-11; 
Yawen Xu, Li Wang, Jun Zhu, Ping Jiang, Zhan Zhang, Lei Li, Qian Wu
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Proteins, Expression, Isolation and Analysis … The primers used in this study were synthesized by GenScript Biotech Corp (Nanjing, China) (Table S1). PCR was performed in 10 μL reaction volume on a LightCycler? 96 Detection System (Roche Diagnostics (Shanghai) Ltd., Switzerland) according to the manufacturer's … Get A Quote

摘要

background: Recently, both trivalent chromium Cr (III) and hexavalent chromium Cr (VI) have been reported to produce neurotoxicity. However, the underlying mechanisms of the neurotoxicity caused by different chemical valence of chromium remain unclear. objective: The purpose of this study was to investigate the mechanism of neurotoxicity induced by exposure to chromium with different valence states based on metabolic disturbance in zebrafish larvae. methods: Zebrafish embryos were exposed to 1?mg/L Cr (III) and 1?mg/L Cr (VI) for 120 hpf respectively. The related indexes of neural development were observed by stereoscope and behavior analysis system. 8OH-dG were detected using enzyme-linked immunosorbent assa... More

關鍵詞

Chromium, DNA methylation, Histone methylation, Metabolism, Neurotoxicity, One carbon metabolism, Zebrafish
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