| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Four leaflets on the third leaf of 4-week-old plants were first syringe infiltrated with 1 x 108?cfu/mL of heat-killed DC3000ΔavrPtoΔavrPtoBΔhopQ1-1ΔfliC?(DC3000ΔΔΔΔ) complemented with a?fliC?allele from DC3000 or ES4326, or no?fliC?(empty vector; EV). Sixteen hours later, the whole plants were vacuum inoculated with DC3000ΔavrPtoΔavrPtoBΔfliC?(DC3000ΔΔΔ) at 5 x 104?cfu/mL. Bacterial populations were measured two days after inoculation. Alternatively, plants were first syringe infiltrated with 1 μM flg22 (GenScript), 1 μM flgII-28 (EZBiolab), or buffer alone (10 mM MgCl2), respectively. Plants were inoculated with DC3000ΔΔΔ 16 h later and bacterial populations were measured two days after inoculation as described above. | Get A Quote |
Wall-associated kinases (Waks) are known to be important components of plant immunity against various pathogens including?Pseudomonas syringae?pv. tomato (Pst) although their molecular mechanisms are largely unknown. In tomato,?SlWak1?has been implicated in immunity because its transcript abundance increases significantly in leaves after treatment with the flagellin-derived peptides flg22 and flgII-28, which activate the receptors Fls2 and Fls3, respectively. We generated two?SlWak1?tomato mutants (Δwak1) using CRISPR/Cas9 and investigated the role of?SlWak1?in tomato-Pstinteractions. PTI activated in the apoplast by flg22 or flgII-28 was compromised in Δwak1 plants but PTI at the leaf surface was una... More
